HEAT-RESISTANT NON-REDUCING CARBOHYDRATE-PRODUCING ENZYME AND ITS PRODUCTION AND USE

摘要

PURPOSE: To obtain a new enzyme derived from Sulfolobus microbes, capable of producing a non-reducing carbohydrate having trehalose structure at the terminal from a reducing starch partially decomposed product, thus capable of giving heat-resistant non-reducing carbohydrates useful for foods/beverages, cosmetics, medicines etc. CONSTITUTION: Microbes having ability to produce an enzyme capable of producing a heat-resistant non-reducing carbohydrate having trehalose structure at the terminal from a reducing starch partially decomposed product >=3 in degree of polymerization of glucose (e.g. Sulfolobus acidocaldarius ATCC 33909) are cultured in a trophic medium, and the product is taken from the cultured product, thus obtaining the objective new enzyme having the above-mentioned activity and the following characteristics: molecular weight: about 69000-79000 dalton (SDS-PAGE); isoelectric point: pI about 5.4-6.4 (ampholine-contg. electrophoresis); optimal temperature: about 75 deg.C in a reaction at pH5.5 for 60min; optimal pH: 5.0-5.5 in a reaction at 60 deg.C for 60min.