| 摘要 |
PURPOSE:To produce indigo for dark blue dye in high efficiency by growing microorganism capable of producing an alkene monoxygenase enzyme activity in a medium incorporated with indole, converting the indole to indigo by oxidation and collecting the product. CONSTITUTION:An indigo which is a dark blue dye for dyeing mainly cellulose fiber to dark blue color is produced in high efficiency by inoculating a microbial strain capable of producing an alkene monoxygenase enzyme [e.g. Nocardia corallina B-276 (FERM P-4094)] to a medium incorporated with indole, culturing at 30 deg.C for 12hr to effect the microbiological oxidation of indole to indigo by the microorganism, extracting and collecting the dye produced and accumulated in the medium by using chloroform as the extraction solvent and purifying the dye by silica gel column chromatography. |
