| 摘要 |
Polypeptides with saponin glycosyl hydrolase activity, including avenacinase and tomatinase, have been purified and encoding nucleic acid therefor cloned and manipulated. The nucleic acid is useful in production of the polypeptides, for example in host cells such as plant or microbial cells, and in screening for other saponin glycosyl hydrolase genes. Oligonucleotides for use in probing have been designed using sequence information obtained. The polypeptides may be used in deglycosylating saponins, in raising antibodies which may be used in screening for other saponin glycosyl hydrolases, and in identifying molecules able to modulate saponin glycosyl hydrolase activity, which molecules are themselves useful. Polypeptides with saponin glycosyl hydrolase activity, and encoding nucleic acid therefor, have been obtained from Gaeumannomyces graminis var. tritici and graminis. |
