NOVEL HYBRID MICROORGANISM

摘要

The fermentation of demethyl tetracycline (I) with hybrid of Streptomyces aureofaciens (II) CKD 3562 (KCTC 8526P) (III) in the media of starch 5.5%, cotton seed powder 2%, calcium carbonate 0.6%, yeast extract 0.13%, vegetable oil 0.5%, casein 0.1%, ammonium chloride 0.2%, trace elements at pH 7.0 yields 3750 mg/l. Treated (II) ATCC 12551 with UV, his requiring mutant and arg requiring mutant are selected, and the protoplasts of each mutant are fused by PEG method. (III) can grow without inhibition by chloride and copper used for conversing (I) into minocycline in antibiotic.