| 摘要 |
The present invention relates to constructing expression vector pSTE-SAK-1 by using staphylokinase amplified by PCR and prokaryotic expression vector, such as PLY-4, and transforming E. coli with pSTE-SAK-1, and inducing the expression of such gene by heating. The engineered bacteria are propagated by fermenting, and destructed. The r-SAK product is purified from the supernatant by a two-step method which includes ion exchange and gel filtration; the purity and the yield of the staphylokinase of the invention are high, and cost low.
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