发明名称 |
Reagenz und Verfahren zur Verwendung desselben in der automatischen durchflusszytometrischen Bestimmung von mindestens einer Leukocyten-Subpopulation aus Vollblut. |
摘要 |
The present invention relates to a reagent and to a method of using the same for automatically determining at least one leukocyte sub-population from a total blood sample. Using this reagent, it is possible, in a single step, to lyse the erythrocytes through the action of saponin and SDS, as well as to ensure the differential staining of the leukocyte sub-populations, at the same time preserving the integrity and morphology of the cells, through the combined action of various components including chlorazol black, an alcohol, a surfactant and preserving agents. The reagent according to the invention can be applied to different types of automatic flow cytometry analyzers. |
申请公布号 |
DE69016377(T2) |
申请公布日期 |
1995.05.24 |
申请号 |
DE1990616377T |
申请日期 |
1990.11.15 |
申请人 |
ABX, MONTPELLIER, FR |
发明人 |
LEFEVRE DIDIER, LEFEVRE DIDIER, F-78200 MANTES LA VILLE, FR;CHAMPSEIX HENRI, CHAMPSEIX HENRI, F-78360 MONTESSON, FR;VOLTAS NADINE, VOLTAS NADINE, F-33000 BORDEAUX, FR |
分类号 |
G01N33/49;C12Q1/06;G01N33/50;(IPC1-7):G01N33/50 |
主分类号 |
G01N33/49 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|