PRODUCTION OF INDIGO BY ENZYMIC METHOD

摘要

PURPOSE:To efficiently obtain indigo according to an enzymic method by prewashing a microbial cell which belongs to the genus Pseudomonas and has the ability to produce the indigo with an aqueous solution containing sodium chloride and then reacting the resultant washed microbial cell or its treated substance with an aqueous solution containing indole. CONSTITUTION:A microbial cell which belongs to the genus Pseudomonas and has the ability to produce indigo [e.g. Pseudomonas sp. MY-6 strain (FERM P-11963)] is inoculated into a culture medium and cultured at 30 deg.C for 24hr by shaking and the resultant culture solution is then centrifuged to collect the microorganism. The collected microbial cell thereof is then washed by performing the operation to suspend the microbial cell in an aqueous solution containing sodium chloride, centrifuge the suspension and collect the microbial cell. The microbial or its treated substance is subsequently suspended in an aqueous solution containing indole, placed in a ventilating stirrer and made to react at 30 deg.C for 48hr. Thereby, the indigo useful as an industrial dye can efficiently be obtained from the indole in high yield by using the enzymic method.