发明名称 |
Dyes designed for high sensitivity detection of double-stranded DNA |
摘要 |
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
|
申请公布号 |
US5312921(A) |
申请公布日期 |
1994.05.17 |
申请号 |
US19930060910 |
申请日期 |
1993.05.14 |
申请人 |
REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
GLAZER, ALEXANDER N.;BENSON, SCOTT C. |
分类号 |
G01N33/533;C07D215/38;C07D221/12;C07D405/12;C07D417/06;C07D417/14;C07H21/04;C09B23/04;C09B69/00;C09K11/06;C12N15/09;C12Q1/68;G01N27/447;(IPC1-7):C07D215/38 |
主分类号 |
G01N33/533 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|