发明名称 |
Method for measuring heparin |
摘要 |
A method for assaying body fluid samples containing heparin and a diagnostic kit are described. Since the reactions go to completion, timing of the assay is not required. The sample is mixed with a heparin-dependent protease inhibitor and either a heparin-independent irreversible inhibitor or a protease substrate. The coagulation enzyme (protease) is then added in a limiting quantity and it either distributes between the heparin-dependent inhibitor and the heparin-indepedent irreversible inhibitor, or the heparin-dependent inhibitor and the protease substrate. The distribution pattern of complex formation of the protease with the two inhibitors or the level of product of the protease-catalyzed hydrolysis of the substrate are used as measures of the heparin activity. The irreversible inhibitor is a peptidyl chloromethyl ketone and the substrate is a synthetic chromogenic or fluorogenic compound that produces a readily measured signal.
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申请公布号 |
US5308755(A) |
申请公布日期 |
1994.05.03 |
申请号 |
US19920895078 |
申请日期 |
1992.06.08 |
申请人 |
RESEARCH CORPORATION TECHNOLOGIES, INC. |
发明人 |
NESHEIM, MICHAEL E.;MANUEL, REGINALD P. |
分类号 |
C12Q1/56;G01N33/573;G01N33/86;(IPC1-7):C12Q1/56 |
主分类号 |
C12Q1/56 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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