METHOD FOR PURIFICATION OF KOREAN TYPE HCV-CORE 14 PROTEIN

摘要

A recombinant strain Saccharomyces cerevisiae DC04-UB-CORE (ATCC 74081) carrying recombinant vector pYLBC-A/B-UB-CORE 14 was cultured in a leucine deficient medium containing 4 % glucose for 12 hrs at 30 deg.C. The preculture was inoculated to a YEPD medium containing 2 % glucose and culture for 24 hrs with shaking. The cell pellet was recovered by centrifugation and added with glass beads. After disruption of cell, insoluble precipitate was recovered by centrifugation. The insoluble precipitate was dissolved in a buffer containing 8 M urea and the specific antigen KHCV CORE 14 was purified from the solution by CM-Sepharose ion-exchange chromatography and S-200 gel filtration chromatography.