| 摘要 |
<p>Nucleic acid analogues of the kind described in PCT/EP92/01220 (PNA's) which hybridise strongly to nucleic acids are used to inhibit nucleic acid amplification procedures such as PCR. False positives in subsequent PCR assays are prevented by hybridising a PNA to PCR amplification products. Assays capable of discriminating between single base pair mutants are conducted by using a PNA hybridising to one of the two allelic forms to inhibit a PCR amplification of that form selectively. Asymmetric PCR amplifications are carried out by starting a PCR symmetricaly using like quantities of forward and reverse primers and once the amplification is established disabling one primer by hybridising a PNA thereto.</p> |
