| 摘要 |
<p>Process for RNA synthesis consisting in successively condensing nucleosides of the formula (I) in which R1 is a puric or pyrimidic optionally protected base, R2 is a leaving group such as the dimethoxytrityl radical, R3 is a phosphorus-containing radical, for example, phosphoramidite and R4 is a trialkylsilyl radical. The RNA obtained is then subjected to a protective treatment with a reagent of the formula (II), for example N(C¿2?H5)3, 3HF, to eliminate R?4¿ protective groups of the 2' hydroxyl groups. Such reagent provides full deprotection in about ten hours, with an RNA of great length, for example, having more than 50 bases.</p> |
