DETECTION OF NUCLEIC ACID SEQUENCES USING PARTICLE AGGLUTINATION

摘要

DETECTION OF NUCLEIC ACID SEQUENCES USING PARTICLE AGGLUTINATION A method of detecting, identifying and/or quantitating nucleic acids in a sample through determination of agglutination or inhibition of agglutination of suspendable particles having nucleic acids bound thereto is described. The nucleic acids can be bound directly to the particle surfaces or attached through a spacer molecule which can, in turn, be either covalently bound or adsorbed to the particle surfaces, The suspendable particles are small enough to remain in suspension and will generally have a large particle size relative to the molecular weight of the DNA or RNA attached to the surfaces. The presence or absence of nucleic acid sequences in a sample is determined by detecting agglutination or inhibition of agglutination of particles having bound thereto nucleic acid sequences complementary to those of interest in the sample.