PATHOGEN DETECTION

摘要

The present invention provides a method of detection of target cells in a complex mixture wherein the said mixture is contacted with magnetic particles carrying one or more binding partners binding specifically to the target cells whereby the magnetic particles are bound selectively to the target cells, followed by magnetic aggregation of the magnetic particles and cells and separation thereof from the complex mixture, liberation of DNA and/or RNA from target cells, so separated, amplification of DNA characteristic of said cells by the polymerase chain reaction (PCR) and detection of the amplified DNA, and a test kit for carrying out such a method. The method of the invention may be used for the detection of pathogenic bacteria in clinical samples and foods.