Pyruvate oxidase mutants, DNA expressing pyruvate oxidase and methods of use thereof

摘要

The present invention provides a pyruvate oxidase which decarboxylates pyruvate to form inter alia hydrogen peroxide and is active without the addition of FAD, thiamine pyrophosphate and divalent metal ions. The amino acid sequence of the enzyme changes at least one proline in position 178 and alanine in position 458 to a different amino acid. The present invention also provides a process for the preparation of this pyruvate oxidase and methods of use thereof.