CLONING AND EXPRESSION OF PROTEIN ANTIGEN OF TOXOPLASMA GONDII

摘要

2098845 9211366 PCTABS00014 A purified antigen of T. gondii having a molecular weight of 54-kDa (as determined by Western blotting) is provided which is reactive with antibodies raised against a recombinant protein obtained by expression of a defined T. gondii cDNA (Figure 2) in a host cell; also provided are immunogenic fragments of the 54-kDa antigen. Recombinant DNA encoding the 54-kDa antigen, host cells for expression of such DNA, a vaccine for treating or preventing toxoplasmosis infection in humans and animals, and test kits for detection of T. gondii antigen or antibody are also described.