| 摘要 |
<p>An on-line multidimensional chromatographic system (10) and method which couples a liquid chromatograph (12) to a supercritical fluid chromatograph (58). The system (10) includes an inlet column (36) for depositing a fraction of interest eluting from the liquid chromatograph (12), an interface (54) interposed between the inlet column and the supercritical fluid chromatograph (58) for receiving and trapping an analyte from the deposited fraction on an impacted surface (74), and a valve arrangement (18, 20, 22, 24) for controlling fluid flow throughout the system (10). After the fraction of interest has been deposited in the inlet column (36), the liquid chromatograph solvent is removed from the inlet column (36), and then the deposited fraction is extracted from the inlet column (36) in a supercritical fluid stream. The supercritical fluid stream is directed into the interface (54), where the analyte is trapped while the supercritical fluid is decompressed and vented. A subsequent supercritical fluid stream is then used to introduce the trapped analyte directly into the analytical column (56) of the supercritical fluid chromatograph (58).</p> |
