摘要 |
<p>Mercapto compounds are determined by reacting the compound with a peroxidase or thiol oxidase reductase in the presence of a chromogen of the formula
<CHEM>
wherein Y is hydrogen or hydroxyl; R1, R2 and R3 may be the same or different, and are groups represented by the following general formula (II), (III) or (IV):
<CHEM>
wherein Z (which may be the same or different) is hydroxyl, amino, substituted amino, alkyl, substituted alkyl, alkenyl, aryl, substituted aryl, acyl, halogen, nitro, sulfo, carboxyl or alkoxy; n is 0, 1, 2 or 3; provided that at least one Z in R1, R2 and R3 is hydroxyl, amino, or substituted amino.</p> |