表达酶原形式的人蛋白C的载体和化合物

摘要

本发明描述了一种重组产生酶原型人蛋白C的方法，所述酶原型蛋白C不同于天然酶原蛋白C，它们对凝血酶和凝血酶/凝血调变蛋白的活化作用更为敏感。本发明还公开了该方法所用的DNA化合物、载体和转化体。

主权项

1、一种制备重组DNA载体的方法，包括将(Ⅰ)重组DNA克隆载体和(Ⅱ)含有一种蛋白质编码顺序的DNA化合物相连接，所述蛋白质从氨基端到羧基端含有： a)一种γ-羧基化的分泌蛋白的信号肽和前肽； b)人蛋白C轻链； c)选自赖氨酸-精氨酸、精氨酸-赖氨酸、赖氨酸-赖氨酸及精氨酸-精氨酸的二肽；及 d)氨基酸残基顺序： ASP THR GLU R1R2R3R4R5R6R7R8R9R10R11R12GLY LYS MET THR ARG ARG GLY ASP SER PRO TRP GLN VAL VAL LEU LEU ASP SER LYS LYS LYS LEU ALA CYS GLY ALA VAL LEU ILE HIS PRO SER TRP VAL LEU THR ALA ALA HIS CYS MET ASP GLU SER LYS LYS LEU LEU VAL ARG LEU GLY GLU TYR ASP LEU ARG ARG TRP GLU LYS TRP GLU LEU ASP LEU ASP ILE LYS GLU VAL PHE VAL HIS PRO ASN TYR SER LYS SER THR THR ASP ASN ASP ILE ALA LEU LEU HIS LUE ALA GLN PRO ALA THR LEU SER GLN THR ILE VAL PRO ILE CYS LEU PRO ASP SER GLY LEU ALA GLU ARG GLU LEU ASN GLN ALA GLY GLN GLU THR LEU VAL THR GLY TRP GLY TYR HIS SER SER ARG GLU LYS GLU ALA LYS ARG ASN ARG THR PHE VAL LEU ASN PHE ILE LYS ILE PRO VAL VAL PRO HIS ASN GLU SER GLU VAL MET SER ASN MET VAL SER GLU ASN MET LEU CYS ALA GLY ILE LEU GLY ASP ARG GLN ASP ALA CYS GLU GLY ASP SER GLY GLY PRO MET VAL ALA SER PHE HIS GLY THR TRP PHE LEUVAL GLY LEU VAL SER TRP GLY GLU GLY CYS GLY LEU LEU HIS ASN TYR GLY VAL TYR THR LYS VAL SER ARG TYR LEU ASP TRP ILE HIS GLY HIS ILE ARG ASP LYS GLU ALA PRO GLN LYS SER TRP ALA PRO-COOH 其中R1选自ASP和LEU、R2选自GLN和HIS，R3选自GLU和LYS，R4选自ASP和LEU，R5为GLN，R6选自VAL和THP，R7选自ASP、PHE和TYR，R8为PRO，R9为ARG，R10选自LEU和THR，R11为ILE或缺失，R12为ASN，-COOH为羧基端。