发明名称 Intronic overexpression vectors
摘要 DNA constructs are provided employing intronically positioned expression, systems, where one of the genes is a dominant gene, usually amplifiable, and the other gene encodes a sequence of interest. Higher levels of co-expression are achieved than when the genese are ligated in tandem. Specifically, the gene of interest is inserted into the intron of a DHFR minigene, the construct transformed into a mammalian cell and the resulting transformants stressed with progressively increasing levels of methotrexate. Substantially increasing levels of co-expression are achieved with increasing levels of methotrexate.
申请公布号 US5043270(A) 申请公布日期 1991.08.27
申请号 US19890331434 申请日期 1989.03.31
申请人 THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY 发明人 ABRAMS, JOHN M.;SCHIMKE, ROBERT T.;THORPE, SUSAN M.
分类号 C12N15/85 主分类号 C12N15/85
代理机构 代理人
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