METHOD FOR QUANTITATIVELY MEASURING COLLAGENASE

摘要

2074507 9111714 PCTABS00006 A solid phase immunoassay using a natural protease substrate as solid phase to capture a metalloproteinase enzyme capable of degrading gelatin and type IV collagenase is provided. Gelatin is bound to a solid substrate and soluble type IV collagenase of interest is introduced. After washing and introduction of affinity purified, rabbit anti-peptide antibodies against type IV collagenase followed by goat anti-rabbit-peroxidase conjugate, the amount of bound enzyme is determined.