SUBSTANTIALLY HOMOGENEOUS 2,5-DKG REDUCTASE, THE PREPARATION THEREOF AND USE IN CONVERSION OF 2,5-DIKETO-D-GLUCONIC ACID INTO 2-KETO-L-GULONIC ACID

摘要

A protein having 2,5-diketogluconic acid (2,5-DKG) reductase activity is produced by recombinant host cells. This can be used to convert 2,5-DKG stereoselectively into 2-keto-L-gulonic acid (2-KLG) which is a useful intermediate in the production of ascorbic acid (vitamin C).