发明名称 |
PH phi 29 DNA polymerase |
摘要 |
An improved method for determining the nucleotide base sequence of a DNA molecule. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA-polymerase which is a phi 29-type DNA polymerase.
|
申请公布号 |
US5001050(A) |
申请公布日期 |
1991.03.19 |
申请号 |
US19890328462 |
申请日期 |
1989.03.24 |
申请人 |
CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS |
发明人 |
BLANCO, LUIS;BERNAD, ANTONIO;SALAS, MARGARITA |
分类号 |
C07H15/12;C07H17/00;C12N9/12;C12N15/09;C12N15/10;C12N15/54;C12P19/34;C12Q1/68;C12Q1/70;G01N33/566 |
主分类号 |
C07H15/12 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|