METHOD FOR STABILIZING HETEROLOGOUS PROTEIN EXPRESSION AND VECTORS FOR USE THEREIN

摘要

The present invention provides a method for stabilizing heterologous protein expression in bacteria by using a 3' truncated chloramphenicol acetyltransferase (CAT) gene fused in frame with a gene encoding a heterologous protein. When expressed in a bacterial host, the resulting hybrid gene produces a fusion protein in recoverable yield. Cleavage sites separating the CAT and heterologous protein are also provided to facilitate isolation and purification of the desired heterologous protein. The invention further provides bacterial vectors containing the hybrid gene fusions for expression of the fusion protein comprising the desired heterologous protein.