VERFAHREN ZUM KONSTRUIEREN VON ARSENRESISTENTEN VEKTOREN FUER THIOBACILLUS FERROOXIDANS

摘要

A process for constructing the plasmids pDR416 and pDR420 which contain arsenic resistance genes and which are able to replicate in both T. ferroxidans and E. coli comprises preparing a recombinant of pBR325 or the like and a T. ferrooxidans plasmid, forming a deletion plasmid and inserting arsenic resistance genes e.g. from R46 by EcoR1 excision and ligation.