| 摘要 |
Detection of hepatitis B (HB) virus comprises (a) dissolving up to 2 kinds of antigen ( or antibody) of HB i.e. HBsAg, anti-HBsAg, anti-HBc, anti-HBcIgM, HBeAg and anti-HBe in buffer soln. of pH 6-9; (b) fixing them on a protein-adsorptive membrane (or sheet, i.e. polystyrene, nitrocellulose or active nylon) to form sepd. spots; (c) reacting with test samples in the mixt. soln. e.g. of enzyme and monoclonal antibody for 30-60 min. at 37-40 deg.C; and (d) washing with water and soaking into 4-chloro-naphthol to develop black or blue spot |
