| 摘要 |
<p>PURPOSE:To obtain eicosapentaenoic acid or docosahexaenoic acid which is a raw material for reagent, medicine, healthy food, etc., in high purity by hydrolyzing a fish oil readily and inexpensively available and used as a raw material with a specific lipase to advantageously afford an intermediate raw material and treating the obtained intermediate raw material in combination with prescribed concentration processes. CONSTITUTION:A fish oil (preferably bluish fish such as sardine or Pacific saury) is hydrolyzed with a lipase derived from the Genus Candida having characteristics hardly hydrolyzing docosahexaenoic acid. The obtained decomposition mixture is divided into a fatty acid and glyceride. Then each ingredient is esterified in the presence of a small amount of a catalyst (e.g., hydrochloric acid or sulfuric acid) in a lower alcohol to afford a fatty acid lower alkylester. Then highly unsaturated fatty acid ingredient is concentrated by an urea addition method and further concentrated and purified by supercritical carbon dioxide gas extraction method or liquid chromatography method to each provide eicosapentaenoic acid from the fatty acid ingredient and docosahexaenoic acid from the glyceride ingredient.</p> |
