| 摘要 |
PURPOSE:To make possible to produce dipeptidyl carboxypeptidase (DPCPase) as dipeptide cutting enzyme from strain having no pathogenicity of the fungus own by culturing microorganism belonging to Bacillus and collecting. CONSTITUTION:For instance, in a medium composing 1% peptone, 0.5% yeast extract and 0.5% salt as a basal medium, Bacillus pumilus HL 721 strain and Bacillus subtilis HL 521 strain producing dipeptidyl carboxypeptidase (DPCPase) belonging to Bacillus having high safety are cultured at 30-45 deg.C for about 16-24 hour. Next, after the culture, fungus body is obtained by centrifugation, suspended by buffer solution and the fungus body is broken by ultrasonic. Further, broken fungus body is removed by centrifugation. Then, DPCPase is obtained by Q-cephalose, hydroxylapatite and gel filtration. By said method, preparation in a large amount is possible and so, dipeptide is effectively produced from any peptide raw material. |
