发明名称 |
GENE SYNTHESIS |
摘要 |
Double stranded DNA (ds-DNA) can be prepared by preparing hybrid DNA containing a single stranded portion and a double stranded portion and carrying out in vivo gap repair on the hybrid DNA. The hybrid DNA is prepared by synthesising a single strand of DNA and introducing the single strand into double stranded DNA. This method of synthesis can be used to make synthetic genes, including synthetic DNA coding for the TAT protein of HIV-I, which includes sequence (I), and incorporates useful restriction sites. Also included are flanking restriction sites to simplify the incorporation of the gene into any desired expression system. |
申请公布号 |
WO8909824(A2) |
申请公布日期 |
1989.10.19 |
申请号 |
WO1989GB00384 |
申请日期 |
1989.04.14 |
申请人 |
BRITISH BIO-TECHNOLOGY |
发明人 |
EDWARDS, RICHARD, MARK;ADAMS, SALLY, ELIZABETH |
分类号 |
C12N15/09;C07K14/16;C12N15/10;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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