| 摘要 |
PURPOSE:To enable the determining of numerous base sequences with one separate electrophoresis pattern, by making a two-way pulse electric field act on a medium for electrophoresis in an electrophoresis for determining a base sequence of nucleic acid. CONSTITUTION:A gel medium film containing a compound containing at least one carbamoil group as polyacrylic amide water gel and a modifier is used as medium film for electrophoresis. A DNA fragment sample is injected into a sample slot of the gel medium film and a DC electric field (high potential on the lower end side) of 35V/cm is made to act on the DNA sample for about 10min to be shifted to a migration start position inside the gel film. Then, pulse electric fields EF and ER in two directions facing each other are applied cyclically to perform an electrophoretic separation. Thus, a band interval expands in a high molecular weight part area to increase the number of readable bases. |
