| 摘要 |
PURPOSE:To simplify measurement by adding hydrogen peroxide and o-phenylene diamine to a specimen soln. contg. an antigen or antibody which is a specimen and contg. a peroxidase labeled antibody or antigen, thereby coloring a fibroin film. CONSTITUTION:The fibroin film to which the antibody (or antigen) to the specimen is included and immobilized is immersed in the specimen soln. contg. the antigen (or antibody) which is the specimen and contg. the peroxidase labeled antibody or antigen to effect an immune reaction. The substrate soln. contg. the hydrogen peroxide and o-phenylene diamine is then added to the soln. to oxidize the o-phenylene diamine by the peroxidase and to color the film to brown. The fibroin film is then taken out of the reaction liquid and the degree of coloring of the film is measured, by which the antigen or antibody quantity is measured. The easy measurement of the specimen quantity is thereby permitted.
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