| 摘要 |
<p>In a simultaneous marking specific binding assay for an analyte in a liquid sample, such as urine during pregnancy or fertile period test, wherein the liquid sample is simultaneously incubated with a first specific binding reagent immobilised on a solid phase carrier surface and with a second specific binding reagent dissolved or dispersed in the liquid sample and which bears a label by means of which the result of the assay can be determined, the improvement that prior to the incubation the second specific binding reagent is contained within a layer (2b) of readily soluble or dispersible solid material superimposed on the solid phase carrier surface (2a) on which the first specific binding reagent is immobilised. When the solid material dissolves or disperses in the sample liquid, the labelled reagent is released into the sample liquid at the same time as the immobilised reagent is exposed to the sample, and the close proximity of the two reagents overcomes the "hook effect" when the analyte is in high concentration in the sample.</p> |
