| 摘要 |
PURPOSE:To efficiently obtain L-valine useful as a medicine, food or feed enriching agent by a fermentation method, by aerobically cultivating a microorganism, belonging to the genus Brevibacterium and having resistance to DL-alpha- aminobutyric acid in a nutrient culture medium. CONSTITUTION:Brevibacterium flavum MJ-233 (FERM-P No.3068) is subjected to ultraviolet light irradiation or chemical agent treatment to provide Brevibacterium flavum MJ-233-AB-41 (FERM-P No. 3812) having resistance to DL-alpha-aminobutyric acid. The resultant strain is the aerobically cultivated in a culture medium prepared by adding 0.1-5wt%, preferably 1-3wt% DL-alpha- aminobutyric acid to a culture medium containing 1-5vol%, preferably 2-3vol% carbon source, e.g. glucose, etc., as well as a nitrogen source, inorganic salt, if necessary, vitamin, etc., under aerobic condition at pH 5-10, preferably 7-8 and 20-40 deg.C, preferably 25-35 deg.C for 2-8 days, optimally 4-5 days to afford the aimed L-valine. |
