METHOD FOR PURIFICATION OF FILAMENTOUS HEMAGGLUTININ

摘要

: The invention provides an improved method for the purification of filamentous hemagglutinin (F-HA) on an industrial scale which comprises contacting a culture of a microorganism of the genus Bordetella with a cellulose sulfate gel, a polysaccharide gel chemically bound with dextran sulfate, or a crosslinked polysaccharide sulfate gel, thereby adsorbing the F-HA on the gel, and then eluting the F-HA from the gel. The method can give a highly purified F-HA which does not contain any other proteins, lipids, saccharides, etc. and undesirable endotoxins, and hence can be used for producing various reagents, medicines and pertussis vaccine.