| 摘要 |
A method of detecting the presence of a plant antigen in or on a plant, e.g. the detection of Grapevine Virus A, includes immobilising a coating antibody to GVA on a surface, bringing an extract of the grapevine into contact with the coating antibody to allow the GVA which may be present in the grapevine extract to be bound by the coating antibody, bringing a detecting antibody to GVA into contact with the bound GVA to allow the GVA to be bound to the detecting antibody, bringing a bridging antibody into contact with the detecting antibody to allow the bridging antibody to be bound to the detecting antibody, bringing an enzyme anti-enzyme antibody complex (e.g. PAP) into contact with the detecting antibody to allow the enzyme anti-enzyme antibody complex to be bound to the bridging antibody, bringing a substrate for the enzyme into contact with the bound enzyme anti-enzyme antibody complex, and detecting any change of the substrate. This method provides increased sensitivity, reproducibility and simplicity. |
