| 摘要 |
PURPOSE:To exactly make early diagnosis of a redionera disease by refining an anti- redionera bacillus polysaccharide antibody (PS antibody) from the redionera bacillus antibody, sensitizing the same with red blood cells and detecting the polysaccharide antigen contained in the redionera bacillus strain. CONSTITUTION:The polysaccharide is added and mixed to ad with the redionera bacillus antibody and the mixture is centrifugally separated and is washed with a physiological salt soln. The antigen and antibody dissociates and become soluble when an acetic acid buffer soln. is added thereto. Only the antibody settles when a satd. aq. ammonium sulfate soln. is added thereto. The antibody is, therefore, collected by a centrifugal sepn. and the ammonium sulfate is removed, by which the PS antibody is refined. The red blood cells are treated by using a coupling agent consisting of a tannic acid, etc., and a liquid contg. the PS antibody is brought into contact therewith to obtain the PS antibody sensitized blood cells. The sensitized blood cells induce an agglutination reaction when the cells contact the redionera bacillus polysaccharide antigen and therefore, the agglutination reaction is caused by a microtiter method and the concn. of the redionera bacillus polysaccharide antigen in the body fluid is quantitatively determined, by which the early diagnosis of the redionera disease is exactly made.
|
