| 摘要 |
PURPOSE:To mass product a large amount of pure human urogastrone inexpensively, by cultivating a bacterium transformed with a plasmid keeping a specific human urogastrone gene, capable of producing human urogastrone, in a medium. CONSTITUTION:DNA fragment obtained from oligodeoxyribonucleotide shown by formula II is bonded to human urogastrone gene having a base sequence shown by formula I (underlined ATG is translation initiation codon and part in the downstream from the codon is part of DNA sequence to code human urogastrone; underlined AGGAGG is liposome bond site) and the fragment is inserted into a manifestation plasmid vector to give recombinant plasmid PUG12, etc. Then, the plasmid is transduced into Escherichia coli, which is transformed to give a bacterium capable of producing human urogastrone. Then the bacterium is cultivated in a polypeptone medium, etc., at about 37 deg.C for about 2hr to produce human urogastrone. |
