| 摘要 |
PURPOSE:To recover the titled enzyme in high efficiency, by decomposing a cellulosic material with a cellulase, acidifying the liquid obtained by the decomposition and saccharification, adding and dissolving chitosan to the liquid, and adjusting the mixture to alkaline state to effect the deposition and separation of chitosan adsorbing the cellulase. CONSTITUTION:The recovery of cellulase from the liquid obtained by the decomposition and saccharification of a cellulosic material with a cellulase can be carried out by (1) acidifying the liquid after the completion of the decomposition and saccharification process, (2) dissolving chitosan or partially deacetylated chitin in the liquid, (3) adjusting the mixture to alkaline state to effect the precipitation of chitosan or partially deacetylated chitin adsorbing the cellulase, and (4) separating the cellulase-carrying chitosan, etc., together with unreacted solid component.
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