发明名称 |
VERFAHREN ZUR GEWINNUNG VON PHENYLALANIN-DEHYDROGENASE ENTHALTENDEN MIKROORGANISMEN, MIKROORGANISMEN, DIE IN IHNEN ENTHALTENE PHENYLALANIN-DEHYDROGENASE UND DEREN VERWENDUNG ZUR HERSTELLUNG VON L-(ALPHA)-AMINOSAEUREN |
摘要 |
1. Process for obtaining microorganisms containing phenylalanine dehydrogenase by aerobic culture of microorganism-containing samples in aqueous nutrient media which contain a source of carbon, nitrogen and mineral salts and thiamine, characterized in that the samples are first incubated in a nutrient medium with an alkali metal propionate content of 0.1 to 0.5 g/l, colony patterns formed are then transinoculated onto a nutrient medium which contains L-phenylalanine or L-phenylalanine amide as an inducer for the formation of phenylalanine dehydrogenase, the system is incubated and the microorganisms which grow in both nutrient media are selected. |
申请公布号 |
DE3446304(A1) |
申请公布日期 |
1986.07.10 |
申请号 |
DE19843446304 |
申请日期 |
1984.12.19 |
申请人 |
DEGUSSA AG;GESELLSCHAFT FUER BIOTECHNOLOGISCHE FORSCHUNG MBH |
发明人 |
LEUCHTENBERGER,WOLFGANG,DR.;KULA,MARIA-REGINA,DR.;HUMMEL,WERNER,DR.;SCHUETTE,HORST |
分类号 |
C12N1/20;C12N9/06;C12P13/04;C12P13/22;C12R1/01 |
主分类号 |
C12N1/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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