发明名称 VERFAHREN ZUR GEWINNUNG VON PHENYLALANIN-DEHYDROGENASE ENTHALTENDEN MIKROORGANISMEN, MIKROORGANISMEN, DIE IN IHNEN ENTHALTENE PHENYLALANIN-DEHYDROGENASE UND DEREN VERWENDUNG ZUR HERSTELLUNG VON L-(ALPHA)-AMINOSAEUREN
摘要 1. Process for obtaining microorganisms containing phenylalanine dehydrogenase by aerobic culture of microorganism-containing samples in aqueous nutrient media which contain a source of carbon, nitrogen and mineral salts and thiamine, characterized in that the samples are first incubated in a nutrient medium with an alkali metal propionate content of 0.1 to 0.5 g/l, colony patterns formed are then transinoculated onto a nutrient medium which contains L-phenylalanine or L-phenylalanine amide as an inducer for the formation of phenylalanine dehydrogenase, the system is incubated and the microorganisms which grow in both nutrient media are selected.
申请公布号 DE3446304(A1) 申请公布日期 1986.07.10
申请号 DE19843446304 申请日期 1984.12.19
申请人 DEGUSSA AG;GESELLSCHAFT FUER BIOTECHNOLOGISCHE FORSCHUNG MBH 发明人 LEUCHTENBERGER,WOLFGANG,DR.;KULA,MARIA-REGINA,DR.;HUMMEL,WERNER,DR.;SCHUETTE,HORST
分类号 C12N1/20;C12N9/06;C12P13/04;C12P13/22;C12R1/01 主分类号 C12N1/20
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