摘要 |
<p>This assay is a solid phase diffusion technique (25). Unknown, unlabeled test substance molecules (24), are mixed with known concentration of labeled test substance molecules (12). The solid phase (18), has adsorbent molecules (14) bound to it. The test solution is applied with a capillary tube (20) to the solid phase (18) and diffuses outwardly. Diffusion of the test solution through the solid phase (18) continues until all of the labeled test substance molecules (12) and all of the unlabeled test substance molecules (24) are bound to the adsorbent molecules (14). The diffusion pattern of the test solution has a diameter which is greater than the diameter of the diffusion pattern for the labeled test substance alone.</p> |