发明名称 High fusion frequency fusible lymphoblastoid cell line
摘要 Human lymphoblastoid cell line capable of acting as a fusion partner in the preparation of hybridomas is grown and selected under conditions whereby a cell line is obtained having greatly enhanced fusion efficiency over the parent cell line. The cell line is derived from UC 729 by growing in Iscove's modified serum-free medium with plating at relatively high densities, followed by cloning at limiting dilutions and selecting for high frequency fusion. The subject cell line referred to as WI-L2-729 HF2 has the A.T.C.C. designation number CRL 8062, having been deposited on Apr. 2, 1981.
申请公布号 US4594325(A) 申请公布日期 1986.06.10
申请号 US19810247656 申请日期 1981.03.26
申请人 THE REGENTS OF THE UNIVERSITY OF CALIF. 发明人 LUNDAK, ROBERT L.
分类号 C12N15/02;A61K39/395;C07K16/00;C12N5/06;C12N5/16;C12P21/08;(IPC1-7):C12N5/00;C12N15/00;C12R1/91 主分类号 C12N15/02
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