EXPRESSION VECTORS CONTAINING PL PROMOTOR,AND ENGINEERED RESTRICTION SITE FOR CONVENIENT REPLACEMENT OF RIBOSOMAL BINDING SITE,PLASMIDS CONTAINING THE VECTORS,HOSTS CONTAINING THE PLASMIDS AND RELATED METHODS

摘要

A method of producing enzymatically active eucaryotic superoxide dismutase or an analog thereof in a bacterial cell which contains and is capable of expressing a DNA sequence encoding the superoxide dismutase or analog thereof comprising maintaining the bacterial cell under suitable conditions and in a suitable production medium. The production medium is supplemented with an amount of Cu++ so that the concentration of Cu++ in the medium is greater than about about 2 ppm. The invention also concerns methods of recovering purified enzymatically active eucaryotic superoxide dismutase or analogs from bacterial cells producing the superoxide dismutase or analog.