Monoclonal antibodies specific for human hematopoietic cell surface glycoproteins

摘要

Monoclonal antibodies are produced for a family of glycoproteins which are selectively expressed on the surface of nucleated human hematopoietic cells but which are absent from other normal human cells. Mice are inoculated with human hematopoietic cells or fragments thereof, and spleen cells obtained from the mice are fused with murine myeloma cells to produce hybridomas. The hybridomas are cultured as clones, and antibodies obtained from the individual clones are tested for their specificity for surface glycoproteins of nucleated human hematopoietic cells. Antibodies can be obtained from the culture growth medium or from ascitic fluid of mice bearing the hybridoma tumor and can be used to distinguish lymphomas from carcinomas.