发明名称 |
Methods, compositions and elements for the determination of lipase |
摘要 |
A method for the determination of the amount of lipase in a sample comprises the steps of: (a) contacting the sample with a reagent composition comprising: (i) a lipase substrate which is a glycerol triester oil having in one of its two alpha -ester positions a long chain alkyl group having at least 8 carbon atoms and, in its two remaining ester positions, short chain alkyl groups such that, if the long chain alkyl group is hydrolyzed, the resulting diester is water soluble; and (ii) an esterase enzyme capable of catalyzing the hydrolysis of a water soluble glycerol diester to glycerol; and (b) detecting the rate at which glycerol is formed. The compositions of the present invention include the substrate and enzyme as defined. The element of the invention comprises a support having thereon the described composition. The invention is useful for determining lipase in samples which contain endogenous glycerol, such as blood serum and other body fluids.
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申请公布号 |
US4555483(A) |
申请公布日期 |
1985.11.26 |
申请号 |
US19830505049 |
申请日期 |
1983.06.17 |
申请人 |
EASTMAN KODAK COMPANY |
发明人 |
LIMUTI, CHARLES M.;BABB, BRUCE E.;MAUCK, JOHN C. |
分类号 |
C12N9/20;C12Q1/44;(IPC1-7):C12Q1/44;C12N9/18;C12R1/125 |
主分类号 |
C12N9/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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