发明名称 |
METHOD FOR PRODUCING A RECOMBINANT BACULOVIRUS EXPRESSION VECTOR |
摘要 |
A method for producing a recombinant baculovirus expression vector, capable of expression of a selected gene in a host insect cell, is disclosed. The preferred method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant shuttle vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant shuttle vector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome. |
申请公布号 |
ZA8403914(B) |
申请公布日期 |
1985.09.25 |
申请号 |
ZA19840003914 |
申请日期 |
1984.05.23 |
申请人 |
THE TEXAS A & M UNIVERSITY SYSTEM |
发明人 |
GALE E. SMITH;MAX D. SUMMERS |
分类号 |
C07K14/01;C07K14/52;C12N15/00;C12N15/866;C12P21/00 |
主分类号 |
C07K14/01 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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