7-Amino-4-trifluoromethylquinolone derived substrates and method for determining enzymes and inhibitors

摘要

Substrates and their corresponding pharmaceutically acceptable acid salts derived from 7-amino-4-trifluoromethyl-quinolone are disclosed. These substrates are useful for determining the presence of an enzyme or enzyme inhibitor in a biological fluid, wherein the fluid is contacted with a substrate. This mixture is thereafter incubated to effect enzymatic hydrolysis. Subsequently, the presence of the free 7-amino-4-trifluoro-methylquinolone is fluorometrically detected.