| 摘要 |
Process for the definite cross-linking of DNA fragments by means of cohesive ends by replacing a first fragment by a linkage DNA, dividing the obtained DNA in the region of the linkage sequence during the formation of the cohesive end and cross-linking the obtained DNA having the cohesive end with a second DNA fragment, the cohesive ends of the first end of the second DNA fragment being complementary. As linkage DNA, an oligonucleotide is used which has the formula R<1> - (X)n - R<2> wherein R<1> and R<2> represent, reading from left to right, identical tetranucleotide sequences, in themselves complementary, having the formula (II), or R<1> is a pentanucleotide sequence having the formula (III) and R<2> is a pentadesoxynucleotide sequence having the formula (IV), the central nucleotides being complementary sequences of R<1> and R<2> groups being identical, or else R<1> representing a tetranucleotide or pentanucleotide sequence which is not complementary in itself or an hexanucleotide sequence having the formula (V), (VI) or (VII) and R<2> a complementary nucleotide sequence enantiomorphous of R<1> and of the same length. X is a short intermediary compound having the form of an oligonucleotide comprising from 2 to 30 nucleotide units. |
