Immunogens of papilloma viruses.

摘要

This invention describes a process for the production of proteins related to viral capsid proteins of papillomavirus (PV), and to processes and compositions for making and using novel DNA sequences, plasmids and microorganisms to produce the viral proteins. The present invention utilizes recombinant DNA techniques to insert DNA sequences coding for a capsid protein of bovine papillomavirus (BPV), or portions thereof, into DNA vectors, such that the vector is capable of replicating and directing expression of the BPV DNA in a bacterial host or other single cell system. The resulting recombinant DNA molecule is used to transform host cells and thereby enables production of BPV-related proteins. The proteins so produced are isolated, purified and formulated for use as immunogens in vaccines which protect against infection by papillomavirus.