| 摘要 |
PURPOSE:To enable the simple and quick determination of a trace of calmoduline by causing calmoduline and enzyme marked calmoduline to competitively react with a calmoduline antibody insolubilizing carrier to imeasure an enzyme activity. CONSTITUTION:The calmoduline obtained from the cerebrum of a cow is immunized in a rabbit and a calmoduline antibody is prepd. The antibody is adsorbed on a silicone resin body and is insolubilized. On the other hand, an SH group is introduced into the calmoduline, whereafter beta-galactosidase is caused to react with the SH group, and the enzyme marked calmoduline is obtd. The sample of body fluid, the above-described insolubilized calmoduline and the marked calmoduline are mixed to cause competitive reaction with each other. The enzyme activity of the marked calmoduline coupled with the insolubilized calmoduline antibody is measured by adding 4-methyl umbelliferyl-beta-D-galactosid and measuring the fluorescence thereof, whereby the quick determination of a trace of the calmoduline in the body fluid is made possible. Thus, the method is made helpful for elucidation of the interstitial distribution of the calmoduline and diseases. |
