发明名称 |
A method of cleaving double stranded DNA. |
摘要 |
<p>A method of cleaving double stranded DNA at any given point which comprises: (a) converting the double stranded DNA to single-stranded DNA in a region surrounding the intended cleavage site; (b) hybridizing to the single-stranded region formed in step (a) a complementary primer length of single-stranded DNA, the 5 min end of the primer lying opposite the nucleotide adjoining the intended cleavage site; (c) restoring that portion of the second strand eliminated in step (a) which lies in the 3 min direction from said primer by reaction with DNA polymerase in the presence of adenine, thymine, guanine and cytosine-containing deoxynucleotide triphosphates; and (d) digesting the remaining single-stranded lenght of DNA which protrudes beyond the intended cleavage point.</p> |
申请公布号 |
EP0086548(A2) |
申请公布日期 |
1983.08.24 |
申请号 |
EP19830200301 |
申请日期 |
1981.03.23 |
申请人 |
GENENTECH INC |
发明人 |
KLEID DENNIS G;YANSURA DANIEL G;HEYNEKER HERBERTL;MIOZZARI GIUSEPPE F |
分类号 |
C12N15/00;A61K38/00;A61K38/27;A61K38/28;A61K39/00;A61K39/395;C07D249/08;C07D253/06;C07D501/20;C07H21/00;C07H21/04;C07K14/61;C07K14/655;C12N;C12N1/20;C12N15/09;C12N15/10;C12N15/62;C12N15/66;C12N15/71;C12P19/34;C12P21/00;C12Q1/68;(IPC1-7):12N15/00 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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